| dc.description.abstract | The stable chromogenic radical 1,10
-diphenyl-2-picrylhydrazyl (DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular
flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using
one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were
optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell
using the average luminosity of the sensing zone captured as the mean of red, green, and blue (RGB)
value using ImageJs program. By using this RGB value as sensor response, it is possible to determine
antioxidant capacity in the range 1–25 ppm as gallic acid equivalent (GAE) with the response time of
9 min. The reproducibility of sensor was good (RSDo1%) with recovery at 93%–96%. The antioxidant
sensor was applied to the plant extracts, such as sappan wood and Turmeric Rhizome. The results are
good when compared to the same procedure using a UV/Vis spectrophotometer. | en_US |
