Xylanase From Xylanolytic Bacteria Induced By Utilizing Vinasse Wastes

Abstract

The production of xylanase by using vinasse waste as carbon source was studied. Isolate MK-20, identified as xylanolytic bacteria can grow aerobically on vinasse without any nutrients added. During the cultivation, this isolate released xylanase and capable produced xylose from hydrolization of oat spelt xylan. The optimum for xylanase production was obtained after 3 days cultivation at 300C. The enzyme was purified and characterized to have molecular weight of 88.2 KDa, respectively. The enzyme stable in pH 4-8 and temperature below 550C while optimum activity at pH 5 and temperature 500C.