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dc.contributor.authorSARI, Desi Sandra
dc.contributor.authorLATIEF, Fourier Dzar Eljabbar
dc.contributor.authorFERDIANSYAH, Ferdiansyah
dc.contributor.authorSUDIANA, Ketut
dc.contributor.authorRANTAM, Fedik Abdul
dc.date.accessioned2022-03-15T02:30:15Z
dc.date.available2022-03-15T02:30:15Z
dc.date.issued2021-08-10
dc.identifier.govdocKODEPRODI1610101#Kedokteran Gigi
dc.identifier.govdocNIDN0015127804
dc.identifier.urihttp://repository.unej.ac.id/xmlui/handle/123456789/105922
dc.description.abstractThe tissue engineering approach for periodontal tissue regeneration using a combination of stem cells and scaffold has been vastly developed. Mesenchymal Stem Cells (MSCs) seeded with Bovine Teeth Scaffold (BTSc) can repair alveolar bone damage in periodontitis cases. The alveolar bone regeneration process was analyzed by micro-computed tomography (µ-CT) to observe the structure of bone growth and to visualize the scaffold in 3-Dimensional (3D). The purpose of this study is to analyze alveolar bone regeneration by µ-CT following the combination of MSCs and bovine teeth scaffold (MSCs-BTSc) implantation in the Wistar rat periodontitis model. Methods. MSCs were cultured from adipose-derived mesenchymal stem cells of rats. BTSc was taken from bovine teeth and freeze-dried with a particle size of 150-355 µm. MSCs were seeded on BTSc for 24 hours and transplanted in a rat model of periodontitis. Thirty-five Wistar rats were made as periodontitis models with LPS induction from P. gingivalis injected to the buccal section of interproximal gingiva between the first and the second mandibular right-molar teeth for six weeks. There were seven groups (control group, BTSc group on day 7, BTSc group on day 14, BTSc group on day 28, MSCs-BTSc group on day 7, MSCs-BTSc group on day 14, MSCs-BTSc group on day 28). The mandibular alveolar bone was analyzed and visualized in 3D with µ-CT to observe any new bone growth. Statistical Analysis. Group data were subjected to the Kruskal Wallis test followed by the Mann-Whitney (p <0.05). The µ-CT qualitative analysis shows a fibrous structure, which indicates the existence of new bone regeneration. Quantitative analysis of the periodontitis model showed a significant difference between the control model and the model with the alveolar bone resorption (p <0.05). The bone volume and density measurements revealed that the MSCs-BTSc group on day 28 formed new bone compared to other groups (p <0.05). Administration of MSCsBTSc combination has the potential to form new alveolar bone.en_US
dc.language.isoenen_US
dc.publisherJournal of Biomimetics, Biomaterials and Biomedical Engineeringen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectBovine Teethen_US
dc.subjectScaffolden_US
dc.subjectRegenerationen_US
dc.subjectAlveolar Boneen_US
dc.subjectPeriodontitisen_US
dc.subjectMicro-computed tomographyen_US
dc.subject3-Dimensionalen_US
dc.titleMicro-Computed Tomography Analysis on Administration of Mesenchymal Stem Cells - Bovine Teeth Scaffold Composites for Alveolar Bone Tissue Engineeringen_US
dc.typeArticleen_US


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