| dc.description.abstract | Proteins hydrolyzed from melinjo seeds (Gnetum gnemon) at green
(GM), yellow (YM) and red (RM) stages of maturity were studied for their
effectiveness in antioxidant and antidiabetic activities. The seed protein
extract was hydrolyzed using alcalase 2.4L, and the resulting hydrolysates
with the highest degree of hydrolysis, protein profile, and the most potent
contributors to antioxidant and invitro antidiabetic activities were identified.
The degree of hydrolysis value of hydrolysates ranged from 52-84%, and
the SDS-PAGE protein profile showed two distinct bands in which the band
with molecular weight of 30 kDa degraded more intensively. Antioxidant
capacity was measured using different standard methods, including radical
cation 2, 2-azinobis-(3-ethylbenzothizoline-6-sulphonate) (ABTS•+) assay,
hydroxyl radical (OH•
), and superoxide anion (O2
•-) scavenging. The green
hydrolysate (GMH) had significantly higher (p<0.05) free radical scavenging
(ABTS•+, OH•
, and O2•-) activities than that of the yellow hydrolysate (YMH)
and red hydrolysate (RMH). However, invitro antidiabetic testing was
performed based on the inhibitory activity of α-amylase and α-glucosidase.
GMH was found to be more effective than YMH and RMH. These results
showed that the antioxidant and antidiabetic activity in hydrolyzed GM protein
has high potential to be utilized as natural nutraceuticals | en_US |
