Preventive Effect of Catechin Isolate From GMB4 Clone Green Tea Green Tea in Selenite Induced Cataract
Date
2019-11-25Author
CAHYANA, Nugraha Wahyu
WIDJAJANTO, Edi
KALSUM, Umi
PRAYITNANINGSIH, Seskoati
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Show full item recordAbstract
To evaluate the efects of Catechin Isolate From GMB4 Clone Green Tea on
sodium-selenite induced cataract formation. Methods: Cataract Isolate From GMB4 Clone
Green Tea was induced by intraperitoneal injection of 19 µmol/kg sodium selenite to ten dayold Wistar rats. The neonatal rats were randomly divided into five groups (n=5 in each
group): a control group, and four cataract- induction groups, treated with either 0, 50, 100,
200 mg/kg catechin Isolate From GMB4 Clone Green Tea. We performed slit-lamp bio
microscopic analysis, level of GSH and GR. Results: Both eyes of all rats in Group 1 did not
exhibit cataract formation. In Group 2, all rats developed Grade 3 cataract in the lenses of
both eyes. The diference in exhibited cataract in the lens of the right eyes inall rats between
Group 2 and any eyes of groups 3 or 4 were significant (P = 0.001). The mean activities of
GSH and GR in Group 2 rat lenses were significantly lower than the values in lenses of all
rats in Group 1 (P = 0.001, 0.003, 0.001), and in the lenses of the right eyes of rats in Groups
3 and 4 (P = 0.001, 0.020, 0.001). Conclusion: Isolate catechin can efectively prevent
selenite-induced cataract formation. This efect was associated with increased level of
opacity, GSH and GR activities in the lens.
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- LSP-Conference Proceeding [1874]