Ambon Banana-Tree Sap stimulated Endothelial Cell Migration in Human Umbilical Vein Endothelial Cells (HUVECs) Induced with Inflammatory Mediator IL-1
Date
2019-03-04Author
Wulan, Provisia Marthalita Yuning
Dorothy, Maria Juliana
Alinda, Resha
Sofiana, Kristianingrum Dian
Prihardina, Bunga
Permatasari, Nur
Widodo, Moch. Aris
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Show full item recordAbstract
Ambon banana-tree (Musa paradisiaca var.sapientum) sap (ABT sap) contains active
substances
that
can
stimulate
the
wound
healing
process
such
as:
flavonoid,
saponin,
ascorbic acid. Those substances are needed in an important phase of
wound healing called angiogenesis through activation of Vascular Endothelial
Growth Factor (VEGF) pathway that stimulates actin reorganization in endothelial
cell migration and proliferation. The aim of this study is to evaluate the effectiveness
of banana-tree sap in stimulating cells migration, VEGF and nitric oxide
(NO) level in Human Umbilical Vein Endothelial Cells (HUVECs) culture that
induced by pro-inflammatory cytokine IL-1 (Interleukin-1). The ABT sap was obtained
by
pressing
a
fresh
Ambon
banana
stem,
then
was
mixed
with
culture
medium
into 0.125%, 0.25% and 0.5% concentration. The ABT sap then was used
for treating HUVECs cell that induced by IL-1 200 ng/mL for 48-hours. The result
of this study shows that Ambon banana-tree sap increased HUVECs cell migration.
However, after 48-hours treatment with ABT sap showed no difference in the
VEGF and NO level in the culture media. These results suggested ABT sap able
to increase migration to HUVECs that promising for wound healing agent. Therefore,
the
further
research
to
elucidate
the
detailed
mechanism
and
identify
the
active
compound
warrant
to
be
done.
Collections
- LSP-Jurnal Ilmiah Dosen [7287]