The effectiveness of Merremia mammosa (Lour.) extract fractions as diabetic wound healers on diabetic rat model
Date
2018-03-21Author
Sakinah, Elly Nurus
Ulfa, Evi Umayah
Marchianti, Ancah Caesarina Novi
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Introduction: Prevalence of diabetic ulcers in Indonesia ranges from 17.3 to 32.9% of
hospitalized diabetes patients. Approximately 14-24% of them cannot be healed and require
amputation. Treatment of diabetic ulcers is quite difficult, because of the failure of blood
vessels and bacterial infection. Merremia mammosa (Mm) (Lour.) that contains flavonoids are
thought to have potential antioxidant that helps the wound healing process. This study aimed
to determine the effect of Mm(Lour.) extract fractions in wound healing process of diabetic rat
model and searching the most potent fraction in 25 mg effective dose. The dose was proven
effective in other plant fraction and smaller dose in our preliminary study was ineffective.
Method: This experimental study used twenty-five male Wistar rats that were made diabetic
by intraperitoneal injection of 40 mg/kg body weight streptozotocin. Rats divided into five
groups, which consist of positive control (gentamicin 0.1%), negative control (aquadest) and
Mm(Lour.) dose 25 mg each of n-hexane, ethyl acetate and water fraction. Wound was made
by Morton method and treatment applied on the wound every other day for 10 days. Wound
healing process were observed by calculating the percentage of reduction in wound size. Data
were described and analyzed further using appropriate statistic tools.
Results: The percentage of reduction in wound size comparison at day 11 of the incision
showed significant different in every fraction when compared with negative control and no
significant different when compared with positive control. This study showed that among the
three factions, water fraction showed the fastest healing rate (93.4 %).
Conclusion: Mm (Lour.) extract fractions significantly accelerated the process of wound
healing in diabetic rat model and the most effective fraction was water fraction. Therefore, it
is potential to be developed further as a topical drug.
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- LSP-Conference Proceeding [1874]