Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kerusakan Histologis Sel Hati Tikus Wistar yang Diinduksi DMBA (Efek Hepatoprotektif Ekstrak Etanol Brokoli (Brassica oleracea L. var. italica) terhadap Kerusakan Histologis Sel Hati Tikus Wistar yang Diinduksi DMBA)
Date
2018-03-20Author
Kusuma, Muhtar Ady
Sakinah, Elly Nurus
Dewi, Rosita
Metadata
Show full item recordAbstract
DMBA (7,12-Dimethylbenz(α)antracene) is one of Polycyclic Aromatic Hydrocarbon’s form that is
abundant on air pollution, i.e. automobile exhaust, cigarette smoke, and wood stoves. DMBA is
metabolized in liver result in DMBA-DE. DMBA-DE is a free radical compound that has
hepatotoxic effect. Broccoli contains flavonoid that has antioxidant effect. It can stabilize DMBADE
radical
compound.
This
research
was
conducted to investigate the hepatoprotective effect of
ethanolic extract of broccoli on the damage of rat’s liver cells induced by DMBA. This research
used 30 white male rats that was divided into 6 groups: normal control (aquadest), positive
control (DMBA) and the treatment groups(using brocolli ethanolic extract with dose : 250, 500,
1000, and 2000 mg/kgBB). The treatment was given for 7 days followed by single-dose DMBA
15 mg/kgBB induction in the 8
th
day. Histological appearance of liver cells were examined on the
12
th
day. Data were analyzed by using Kruskal Wallis test followed by Mann Whitney. The result
showed that there was a significant difference among the groups with p<0.05. In conclusion,
there were some differences of hepatoprotective effect of broccoli ethanolic extract on the
histologically damage of rats liver cells induced by DMBA.
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- LSP-Jurnal Ilmiah Dosen [7287]