Calprotectin In Gingival Crevicular Fluids Correlates With Clinical Parameter and With The Count of Black-Pigmented Anaerobes Bacteria On The Aggressive Periodontitis

Abstract

Calprotectin is a calcium and zinc-binding cytosolic protein which is expressed in neutrophil during inflammatory event in the initial steps of inflammation. Plasma level of calprotectin, are elevated in patient with bacteria infections, it is exerts profound microbiostatic and modest microbicidal activities such as black-pigmented anaerobes. The black-pigmented anaerobes are found predominantly in deep periodontal pockets. In this study, we investigated the correlation between gingival crevicular fluid (GCF) calprotectin level and clinical indicator (probing depth) and the count black-pigmented anaerobes bacteria. Methods: Probing depth at 12 site of 12 subject with aggessive periodontitis were examined, then GCF sample were collected 2 sterile paperpoints for 30 seconds. The black-pigmented anaerobes grown in the blood agar medium for 7-14 days. Level calprotectin we analysed by ELISA. Results: The GCF calprotectin level increased aproximately in proportion to probing depth (3-7 mm) and showed a significant positive correlated with probing depth (p<0.05). Black-pigmented anaerobes bacteria stimulates calprotectin release from neutrophil, and there were also positive correlations between the total content of calprotectin and the count black-pigmented anaerobes bacteria (r = 0,669, p<0.05) Conclusion: The calprotectin level in GCF correlates well with probing depth and black-pigmented anaerobes bacteria of aggressive periodontitis and suggest that calprotectin may be useful for evaluating the extent of periodontal inflammation.