The Toxicity of Srikaya Seed Granules (Annona squamosa L.) with Different Heating Temperatures Against the Larva of Aedes aegypti L.

Abstract

Aedes aegypti L. contains the virus that causes dengue fever, especially in Southeast Asia. Fogging and using chemicals such as Abate control the mosquito, but on the other hand, it causes Aedes aegypti L. to become resistant. A breakthrough is needed to eradicate the mosquito, which is environmentally friendly, less harmful to non-target animals, and avoid Aedes aegypti L. resistance. The seed of Annona squamosa L. contains annonain and squamosin known to have a toxic effect on the larvae of Aedes aegypti L. Further research is needed to test the heating level of the seed extract granules to find out the more effective treatment to the larvae. This research method uses a completely randomized design with two treatments, six sub treatments, and four repetitions. The temperature of 60 ° C uses concentrations of 1 ppm, 6 ppm, 12 ppm, 18 ppm, 24 ppm, and 30 ppm. As for the temperature of 40 ° C using concentrations of 1 ppm, 7 ppm, 14 ppm, 21 ppm, 28 ppm, and 35 ppm. Each temperature is compared with aquadest and Abate. Data is analyzed using probit analysis to determine the LC50, followed by a paired sample T-test to find out the significant difference. The result showed a higher temperature (60°C) has a higher level of toxicity compared to 40°C.