Celluler Analysis in Orthodontic Tooth Movement Post Robusta Coffee Extract Administration

Abstract

This study aimed to analyze the number of osteoclasts, osteoblasts, fibroblasts, and the improvement of dental movement post Robusta coffee extract administration. 10 guinea pigs were divided into 2 groups i.e. group C: guinea pigs were applied using orthodontic force, and group T: guinea pigs were applied using orthodontic force and 87.5 mg / 500 g BW of Robusta coffee extract. Orthodontic force in guinea pigs was carried out using a orthodontic bracket and open coil spring with strength of 52.5 grams. Observations were conducted on the day 15 by performing histological processing to calculate of the number of osteoblasts, osteoclasts and fibroblasts. The improvement of tooth movement was measured by an X-Ray µ-CT device. Robusta coffee extract increased the number of osteoclasts, osteoblasts, and fibroblasts. The number of osteoclasts in the compression area was greater than that in the tension area (p < 0.05). The number of osteoblasts and fibroblasts in the tension area was greater than that in the compression area (p < 0.05). The Robusta coffee extract also improved the movement of the teeth (p < 0.05). Robusta coffee extract increased the number of osteoclasts, osteoblasts, fibroblasts and improved orthodontic tooth movement.