The Effect of various Sterilization Procedure on Number of Colony-Forming units of isolated endophytic bacteria from Cosmos caudatus Kunth. leaf

Abstract

The purpose of this study was to determine the effect of different concentration and time sterilization on the number of colony-forming units of isolated endophytic bacteria from Cosmos caudatus kunth. leaf. The sterilizing agents were used is sodium hypochlorite (NaOCl). The research consisted of four treatments P1 (NaOCl 5.35%; 3 minutes), P2 (NaOCl 5.35%; 1,5 minutes), P3 (NaOCl 3%; 3 minutes), and P4 (NaOCl 3%; 1,5 minutes). Every treatments have one control medium and three repetition. The control medium treated by make streak using C. caudatus leaf that had sterilized and the three repetition treated by inoculating 100 µL suspension of C. caudatus leaf with 10 -1 serial dilution. Result on the control medium is sterile and indicated that the bacteria in treatments medium is endophytic bacteria. The CFU in treatments P1 (0x10 2 CFU/ml), P2 (2,9 X 10

CFU/ml), P3 (2,9 X 10 3 CFU/ml), and P4 (2,8 X 10

CFU/ml). The conclusion from this study is there was no significantly different of different concentration and time sterilization on the number of colony-forming units of isolated endophytic bacteria from Cosmos caudatus kunth. Leaf.