45 kDa Fimbria Protein of Proteus mirabilis as Hemagglutinin and Adhesion Protein

Abstract

Proteus mirabilis is opportunistic and nosocomial pathogen that usually found in clinical specimen from patients with catheter. The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity of the protein as hemagglutinin and adhesion molecule. The aim of this study is to evaluate the role of 45 kDa fimbria protein from P. mirabilis. After identification, bacterial isolate of fimbria fraction 12,5 % SDS-PAGE were used to isolate fimbria protein followed by hemagglutinin test and in vitro adhesion test. The study showed that the 45 kDa fimbria protein of P mirabilis was a hemagglutinin protein that could agglutinate mice erythrocytes, rabbit erythrocytes, and human group O erythrocytes. Hemaglutination test were negative on erythrocytes human blood group A,B, and AB. The 45 kDa fimbriae protein was also adhesion protein showed by its activity to adherence to rabbit vesica urinaria epithel receptor. The increase dose of 45 kDa fimbriae protein will decrease the amount of P mirabilis bacteria to adherence to rabbit vesica urinaria epithel (p< 0,05 ).