Thymoquinone of Black Cumins Effect towards Socket Bone Formation Process Post Extraction in Diabetic-Induced Rats

Abstract

The objective was to determine the effect of thymoquinone towards socket bone formation process post extraction in diabetic-induced rats. About 27 male Wistar rats were induced diabetes by using streptozotocin (50 mg/b.w) intravenously. Rats with blood glucose level ;,;250 mg/dL were considered DM and were divided into Thymoquinone (Tq), Metformin (Met) and Control (C) group. The treatments were given start from the l st day of DM. On the 7th day after treatment, its mandibular left first molar tooth was extracted. On the 3rd, 7th and 10th day post extraction, 3 rats of each group were euthanized. Blood glucose level was measured before and after diabetes induction, before extraction and euthanized. Paraffin embedding tissue processing method and HE and Mallory's Trichrome staining were used to observing the socket bone healing. T q treatment significantly decreased the blood glucose level on the 10th day post extraction although, it was still above the normal range (334.3±65.9 mg/dL). Histologically, socket bone healing process in Tq group showed a continuously formation of provisional matrix, woven bone and lamellar bone on the 3rd, 7th and 10th-day post extraction compared to Met and C group. The antidiabetic properties of Tq may well-related to socket bone healing and moreover, Tq also may stimulates the osteoblasts activity which the function was essential in bone formation process. Treatment with Tq is effective to prevent the delay of socket bone healing in socket post extraction in diabetic rats by lowering blood glucose level and by stimulating the osteoblasts activity.