Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/86346
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dc.contributor.authorRachmawati, Dessy-
dc.contributor.authorPeferoen, Laura A.N.-
dc.contributor.authorVogel, Daphne Y.S.-
dc.contributor.authorAlsalem, Inas W.A-
dc.contributor.authorAmor, Sandra-
dc.contributor.authorBontkes, Hetty J.-
dc.contributor.authorBlomberg, B. Mary E. von-
dc.contributor.authorScheper, Rik J.-
dc.contributor.authorHoogstraten, Ingrid M. W. van-
dc.date.accessioned2018-07-24T02:51:58Z-
dc.date.available2018-07-24T02:51:58Z-
dc.date.issued2018-07-24-
dc.identifier.issn0165-5728-
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/86346-
dc.descriptionJournal of Neuroimmunology 291 (2016) 89–95en_US
dc.description.abstractOral metal exposure has been associated with diverse adverse reactions, including neurotoxicity. We showed previously that dentally applied metals activate dendritic cells (MoDC) via TLR4 (Ni, Co, Pd) and TLR3 (Au). It is still unknown whether the low levels of dental metals reaching the brain can trigger local innate cells or prime them to become more responsive. Here we tested whether dentally applied metals (Cr, Fe, Co, Ni, Cu, Zn, Au, Hg) activate primary human microglia in vitro and, as a model, monocytic THP-1-cells, in high non-toxic as well as near-physiological concentrations. In addition the effects of ‘near-physiological’ metal exposure on endotoxin (LPS) responsiveness of these cells were evaluated. IL-8 and IL-6 production after 24 h was used as read out. In high, non-toxic concentrations all transition metals except Cr induced IL-8 and IL-6 production in microglia, with Ni and Co providing the strongest stimulation. When using near-physiological doses (up to 10× the normal plasma concentration), only Zn and Cu induced significant IL-8 production. Of note, the latter metals also markedly potentiated LPS responsiveness of microglia and THP-1 cells. In conclusion, transition metals activate microglia similar to MoDCs. In near-physiological concentrations Zn and Cu are the most effective mediators of innate immune activation. A clear synergism between innate responses to Zn/Cu and LPS was observed, shedding new light on the possible relation between oral metal exposure and neurotoxicity.en_US
dc.language.isoenen_US
dc.subjectDentally applied metalsen_US
dc.subjectNeurotoxicityen_US
dc.subjectMicrogliaen_US
dc.subjectTHP-1en_US
dc.subjectInnate immune activationen_US
dc.titleMetal ions potentiate microglia responsiveness to endotoxinen_US
dc.typeArticleen_US
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