PRESERVATION EFFECT OF PEG (POLYETHYLENE GLYCOL) ON SYNTHETIC SEED OF SUGARCANE (Saccharum officinarum) VAR. NXI 1,3

Abstract

Encapsulation is a method for embryonic tissue-coating system using Na-alginate for synthetic seed preservation. In order to produce synthetic seed of sugarcane, coleoptillar embryonic callus and apical bud were encapsulated by encapsulation media (MS0 + 4% Na-alginate + 100 mM CaCl 2 ). In this study, addition of PEG solution (0%, 1%, 2%, 3% (w/v)) in encapsulation media showed desiccation effect. This effect not only resulted in triggered the growth of synthetic seeds but also preserved the synthetic seed. Retarding the growth of synthetic seed is the effect of PEG on preserving the seed. PEG treatment also implied on longer storage periode of the synthetic seed. The result showed that 2% PEG was the most effective concentration of encapsulation media. Desiccation effect of 2% PEG prolonged storage period of synthetic seed until 3 weeks with 89,32 planlets generated and chlorophyll concentration of 26,03 𝝁g/mL.