PENGARUH KETERSEDIAAN OKSIGEN PADA PRODUKSI EPIGLUKAN OLEH Epicoccum nigrum MENGGUNAKAN MEDIA MOLASES

Abstract

The compound β-glucan is a polymer of glucose, which is polymerized through beta glycoside bond. The compound β-glucan has anti-tumor benefits, reducing the risk of heart disease, lowering cholesterol and so forth. The compound β-glucan can be produced by the fungus in ukstraseluler. One of the molds that can produce compounds in the extracellular βglucan is Epicoccum nigrum, often called epiglucan. The purpose of this study was to determine the effect of aeration and oxygenation to the production epiglukan by Epicoccum nigrum in molasses media. This research was conducted using descriptive method with 3 under controlled conditions : anaerobic, aeration and oxygenation in the Stired Continuous Tank Reactor (batch culture). The parameters observed were the biomass (g / L), epiglucan (g/L), reducing sugar content (%) and pellet diameter are formed. Biomass and highest epiglikan obtained in oxygenated conditions which amounted to 7.074 g / L and 2.01 g / L. The resulting biomass pellet-shaped with the largest diameter occurs in conditions of oxygenation that is 0.37 mm. residual reducing sugar at the end of fermentation the greatest there is in the anaerobic condition is 1.529%