Densitometric Determination of Mebhydrolin Napadisylate in Tablets

Abstract

simple and rapid high-performance thin-layer chromatography (HPTLC) densitometric method has been developed for the determination of mebhydrolin napadisylate in tablets. After extraction of the analyte with a mixture of methanol and NH OH 25% (100:1.5, v/v), the extracts were spotted on precoated HPTLC silica gel F254 plates, which were developed with a mixture of methanol and ethyl acetate (1:1, v/v). Quantitative evaluation was performed by measuring the absorbance reflectance of the analyte spots at 287 nm. The method was validated for specificity, linearity, accuracy, precision, and robustness. Good linearity was achieved in the concentration range 600–1600 ng/spot. The RSD of repeatability and intermediate precision were found to be less than 2%, whereas the mean of the recovery data was 99.3–100.8%. The detection limit and quantification limit were 18.5 and 55.5 ng/spot, respectively. The drug was subjected to acidic and alkaline hydrolysis, oxidation, dry heat, and UV treatment. The peak of mebhydrolin napadisylate was not interfered by those of possible degradation products.