KAJIAN EFEK KOPI DAN PERIODONTITIS PADA ATEROSKLEROSIS KORONER

Abstract

Objectives. The objective of this study was to conduct an in vivo experiment to prove the effect of coffee consumption to reduce coronary atherosclerosis in periodontitis rat model. Methods. Twenty one rats (Rattus norvegicus) were divided into three groups, i. e. 1) periodontitis, 2) periodontitis + coffee, 3) control group. Periodontitis rat model was created by means of inserting wire ligature around left molar mandibular tooth followed by injecting periodontitis bacteria Porphyromonas gingivalis in buccal aspect of its teeth twice a week. One dose of decocta coffee (representing one cup) was fed once per day by stomach sondation. The experiment was conducted for 35 days. All rats were fed with normocholesterol standard diet. On the 36th day rats were sacrified. Their hearts which contained coronary arteries were removed and prepared for cross sectional specimens for histopathologic and immunohistopathologic examination. Coronary atherosclerosis markers were intimal thickening, lipids deposit, foam cell and scavenger receptor (Sc-R) expression. Results. Arterial wall of rats that consumed coffee demonstrated symmetric intimal thickness and collagen intimal showed intact and dense. Fewer lipid deposit, smaller number of foam cell and Sc-R expression were identified in coffee group compared to periodontitis group. Conclusions. One cup coffee consumption per day improved the morphology of coronary artery particularly intimal collagen leading to protect against atherosclerosis and rupture. Effect of coffee consumption on collagen vascular is a novel perspective, further studies are needed particularly focusing on this intimal collagen.