Isolasi Kapang Amilolitik Asal Serasah Hutan Pelangi Geoprak Ijen Bondowoso, Jawa Timur

Abstract

Forest litter is formed from complex organic materials in the forest. One of the organic materials is starch. Starch by amylolytic fungi is a source of nutrition for growth. Fungi amylolytic are able to break down starch by producing amylase. The purpose of this research was to isolate, select, and characterize amylolytic molds isolates from litter in the Pelangi Forest Ijen Geopark, Bondowoso East Java. This research method began with the mold isolation stage on PDA media. Furthermore, the selection of amylolytic mold isolates on SSA media with positive parameters based on the formation of a clear zone around the colony. Amylolytic activity was measured based on the amylolytic index. Mold isolates with the highest amylolytic index were characterized and genus identified. The results of molds isolation from litter obtained 34 molds isolates. The results of amylolytic molds selection showed that there were positive isolates for amylolytic. Based on the amylolytic index, it shows that the highest isolates were IHP30, IHP10, and IHP9. The colony characteristics of IHP30 isolate show a bright olive-green color, powdery colony texture, radial lines, and concentric zones and produce exudates. While its microscopic structure shows septate hyphae, the conidiophores have phialids and elongated chains of conidia. Based on its characteristics, isolate IHP30 belongs to the genus Penicillium. The colony characteristics of IHP10 isolate show a forest green color, powdery colony texture, radial lines and concentric zones, and produce exudates. While, the microscopic structure shows septate hyphae, branched conidiophores, phialids, and elongated chain of conidia. Based on its characteristics, isolate IHP10 belongs to the genus Penicillium. The colony characteristics of IHP9 isolate are deep buff color, have a velvety colony texture, have concentric zones but do not have radial lines and produce exudates. While the microscopic structure showed septate hyphae, vesicles, philalids, spreading chain conidia, and unifiber conidia-type morphology. The character of isolate IHP9 shows the genus Aspergillus.