The Role of Protein Pili 95 KDa Shigella dysenteriae as Protein Adhesin in Balb/c Mice Enterocytes

Abstract

Shigella dysenteriae is an obligate pathogen that usually found in clinical specimen from shigelosis patients and causes gastrointestinal tract infections with high morbidity and mortality. The pathogenic mechanism of the bacteria is not fully elucidated especially its potential activity of the pili as hemaglutinin and adhesion molecule. The aim of this study is to predict the molecule weight of pili and OMP from S. dysenteriae. The research stages included identification of S. dysenteriae isolation of OMP S. dysenteriae, sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), hemagglutination test, isolation of enterocyte of BALB/c mice and and adhesion test. The study showed that the molecule weight protein of S. dysenteriae were 155 kDa, 124 kDa, 95 kDa, 78 kDa, and 32 kDa. The protein pili S. dysenteriae with a molecular weight of 95 kDa functions as an adhesin. Changes in pili protein concentration of 95 kDa molecular weight S. dysenteriae coated on the enterocyte of mice Balb/c strain, had a significant effect on the adhesion index. The protein pili S. dysenteriae with a molecular weight of 95 kDa functions as an adhesin.