The Effect of anti miRNA 144 on the Expression of a Globin Chain in PBMC (Peripheral Blood Mononuclear cell) of Major Beta Thalassemia Patients

Abstract

The main pathophysiology basis of E-thalassemia is excess unbound D-globin chain due to reduction D-globin expression. Reduction of D-globin chain can lead clinical improvement. Reduction of D-globin chain is an alternative therapy in E major thalassemia patients. microRNA (miRNA) can regulate D-globin chain through targeting transcription factor. Klfd and GATA-1 erythroid transcription factor regulated by miRNA-144. This study was an experimental study using PBMC of major E thalassemia patient. PBMC divided into two groups that were not transfected and transfected anti-miRNA 144. The expression of miRNA-144 were detected using Exiqon's miRCURY LNATM universal RT microRNA PCR. The expression of D-globin chain protein were detected using immunobloting technique.There are differences in the expression of miRNA-144 who have carried out anti-miRNA 144 transfections with those that have not been conducted transfections in PBMC major E thalassemia patients. There are no differences in the expression of D globin chain who have carried out anti-miRNA 144 transfections with those that have not been conducted transfections in PBMC major E thalassemia patients. Based on this study, the administration of anti-miRNA 144 cannot decrease the expression D globin chain expression in PBMC major E thalassemia patients.