Bioethanol Production from Sugarcane Molasses with Simultaneous Saccharification and Fermentation (SSF) Method using Saccaromyces cerevisiae-Pichia stipitis Consortium

Abstract

The sugar-making industry from sugar cane has a byproduct of sugar cane as molasses. One alternative to reduce the amount of molasses waste is by reusing molasses as biomass for bioethanol production. Molasses can be reused as bioethanol that have economic value because of their high sugar content. The process of making bioethanol from molasses is done by Simultaneous Saccharification and Fermentation (SSF) method using 10% substrate (100 gr / L molasses) and 20% substrat (200 gr / L molasses). The process of hydrolysis of sugarcane molasses is carried out biologically using Aspergillus niger and Trichoderma viride, while the fermentation process uses the consortium Saccaromyces cerevisiae-Pichia stipitis. Ethanol production using the SSF methods had the highest bioethanol concentration of 14.38% v/v for 20% substrat and 10% consortium usage at 72 hours fermentation.