The Effect of Avocado (Persea Americana Mill) Seed Powder on the Lymphocytes Amount in Escherichia Coli Induced Mice (Sprague Dawley)

Abstract

Avocado (Persea americana Mill) is one of the plants that can be used as herbal medicine, while Indonesia is one of the three major avocado producers in the world. All parts of avocado plant can be used as herbal medicine, but until now, it’s still lack of information. In fact, parts of avocado were thrown away as waste, including its seed. On the other hand, the substance contained in avocado seed has pharmacological effect, namely as an analgesic and anti-inflammatory. The aim of this study was to find out the effect of avocado seed powder on the lymphocytes number in Escherichia coli induced Mice (Sprague dawley). A total of twenty-five healthy male mice were divided into five groups respectively to be treated differently for 7 days. Group I was administered sterile distilled water orally as the negative control (without E. coli induction). Group II was administered sterile distilled water orally as the positive control. Group III was administered 5% of avocado seed powder orally. Group IV was administered 10% of avocado seed powder orally. Group V was administered 15% of avocado seed powder orally by means of oral gavage. The blood samples were taken on day 1, 3 and 7. The lymphocytes amount was counted and analysed. Comparing all groups to the one with the negative control, there were significant differences between them, except group IV on day 1. During day 3 and 7 all groups, excluding group IV had significant differences (p<0.05) with the increase of the lymphocytes average amount. In conclusion, 10% of avocado seed powder can effectively suppress the lymphocytes amount in E. coli induced mice.