Immunogenic Proteins from Salivary Gland of Potential Malaria Vector An. vagus and An. sundaicus

Abstract

The salivary gland proteins of the Anopheles (An.) female mosquito play vital roles in the transmission of Plasmodium into the human host. A comprehensive understanding of Anopheles identity and its related salivary proteins is a key for vector-based malaria control. This research aims to analyse the protein profile and immunogenicity of Anopheles salivary gland proteins from 2 potential Anopheles vectors, An. vagus and An. sundaicus. Female Anopheles are collected from Bangsring village, Banyuwangi Regency by direct-landing collection. Each species are identified by morphological features and internal transcribed spacer 2 (ITS2). Immunogenic proteins were determined by Western Blotting with IgG from people living in the endemic area as primary antibodies. Immunoblotting results showed the presence of 3 immunogenic protein bands with a molecular weight of 34, 46, and 66 kDa in both species. Furthermore, the 99 kDa protein band was present merely in An. sundaicus. Moreover, the result showed that two distinct species have different profiles and immunogenic proteins.