Hypoxic Preconditioning For Viable and Self Renewing Mesenchymal Stem Cells (Mscs) As the Regeneration of Spermatogenesis Process

Abstract

The aim of this research was to obtain MSCs that were viable and self renewing for spermatogenesis process by a treatment of hypoxic precondition in vitro culture. In this research, hypoxic precondition was the use of 1% O2 concentration which was compared to those of culture under normoxic (21% O2) condition. Flowcytometric analysis showed that in MSCs culture under 1% O2 concentration, the level of CD90+ and CD34- were not altered (remained undifferentiated), meanwhile under 21% O2 concentration, cells have experienced alteration (became differentiated), that was indicated by the down regulation of CD90+ and up regulation of CD34- . Immunocytochemical and immunofluorescence analysis showed that under 1% O2 concentration, MSCs culture expressed transcription factors, such as OCT4 and SOX2, meanwhile under 21% O2 concentration, the transcription factors OCT4 and SOX2 (self renewal function), were not expressed. In conclusion, this research showed that hypoxic preconditioning with 1% O2 concentration very supported MSCs to remain viable before transplantation for spermatogenesis disorder, because the cells still undifferentiated and self renewal capacity was maintained.