Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/112056
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dc.contributor.authorSEMITA, I Nyoman
dc.contributor.authorUTOMO, Dwikora Novembri
dc.contributor.authorSUROTO, Heri
dc.contributor.authorSUDIANA, I Ketut
dc.contributor.authorGANDI, Parama
dc.date.accessioned2023-02-10T01:27:13Z
dc.date.available2023-02-10T01:27:13Z
dc.date.issued2023-01-01
dc.identifier.govdocKODEPRODI2010101#Pendidikan Dokter
dc.identifier.urihttps://repository.unej.ac.id/xmlui/handle/123456789/112056
dc.description.abstractBackground: Globally, spinal cord injury (SCI) results in a big burden, including 90% suffering permanent disability, and 60%–69% experiencing neuropathic pain. The main causes are oxidative stress, inflammation, and degeneration. The efficacy of the stem cell secretome is promising, but the role of human neural stem cell (HNSC)- secretome in neuropathic pain is unclear. This study evaluated how the mechanism of HNSC-secretome improves neuropathic pain and locomotor function in SCI rat models through antioxidant, anti-inflammatory, anti-matrix degradation, and neurotrophic activities. Methods: A proper experimental study investigated 15 Rattus norvegicus divided into normal, control, and treatment groups (30 µL HNSC-secretome, intrathecal in the level of T10, three days post-traumatic SCI). Twentyeight days post-injury, specimens were collected, and matrix metalloproteinase (MMP)-9, F2-Isoprostanes, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and brain derived neurotrophic factor (BDNF) were analyzed. Locomotor recovery was evaluated via Basso, Beattie, and Bresnahan scores. Neuropathic pain was evaluated using the Rat Grimace Scale. Results: The HNSC-secretome could improve locomotor recovery and neuropathic pain, decrease F2-Isoprostane (antioxidant), decrease MMP-9 and TNF-α (anti-inflammatory), as well as modulate TGF-β and BDNF (neurotrophic factor). Moreover, HNSC-secretomes maintain the extracellular matrix of SCI by reducing the matrix degradation effect of MMP-9 and increasing the collagen formation effect of TGF-β as a resistor of glial scar formation. Conclusions: The present study demonstrated the mechanism of HNSC-secretome in improving neuropathic pain and locomotor function in SCI through antioxidant, anti-inflammatory, anti-matrix degradation, and neurotrophic activities.en_US
dc.language.isoenen_US
dc.publisherKorean Journal of Painen_US
dc.subjectBRAIN-DERIVED NEUROTROPHIC FACTORen_US
dc.subjectF2-ISOPROSTANESen_US
dc.subjectGLIOSIS; MATRIX METALLOPROTEINASESen_US
dc.titleThe Mechanism of Human Neural Stem Cell Secretomes Improves Neuropathic Pain and Locomotor Function in Spinal Cord Injury Rat Models: Through Antioxidant, Anti-Inflammatory, Anti-matrix Degradation, and Neurotrophic Activitiesen_US
dc.typeArticleen_US
Appears in Collections:LSP-Jurnal Ilmiah Dosen

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