Ultrasound-Assisted Extraction of Fucoxanthin from Sargassum Sp: Effect of Extraction Variables to Antioxidant Activity

Abstract

Sargassum sp is a brown algae group that contains fucoxanthin with a molecular structure employing an allenic bond, an epoxy molecule, and a carbonyl group. This structure provides antioxidant activity to fucoxanthin. This study aims to perform fucoxanthin extraction and study the influence of extraction parameters (extraction time, ratio of raw materials to solvents, and particle size) on antioxidant activity. Sargassum sp is fed into a flask containing ethanol. Subsequently, the ultrasound waves are introduced to the flask during the extraction time. The liquid product is filtered off and purified by a rotary evaporator. A total of 17 experiments were conducted on various extraction conditions. This work used the DPPH method to determine antioxidant activity. This work achieved an optimum antioxidant activity at 10 minutes, the ratio of raw materials to solvents was 0.1 g/mL, and the raw material size was 60 mesh. Meanwhile, based on statistical analysis, extraction time is the most influential variable.