The Expression of HSP-60 and MMP=8 on Orthodontic Tooth Movement in the Alveolar Bone after Sodium Fluoriide Administration

Abstract

Applying orthodontic force to teeth causes bone remodeling, namely resorption of alveolar bone in the pressure area of the periodontal ligament, while bone is formed at the tension area. Fluoride is used to stimulate cell bone formation. The purpose of this study is to find out the Sodium Fluoride effect on the HSP-60 and MMP-8 expression in the alveolar bone receiving an orthodontic force of male Wistar rats. The study used a laboratory experimental method that involved 16 male Wistar rats separated into two groups. Group C: rats obtained orthodontic tooth movement and without Sodium Fluoride. Group T: rats received orthodontic strength with Sodium Fluoride 11.75 ppm with topical application. The orthodontic strength used ligature wire with a 0.02 mm wire diameter drawn from the permanent first molars and maxillary incisors. HSP-60 and MMP-8 expression was tested using immunohistochemistry and observed on day 7 and day 14. The results of the data were tested using Anova statistical test to compare variables among groups at a level of significance of 5% (p<0.05). Administration of Sodium Fluoride resulted HSP-60 and MMP-8 as expressed of osteoblast interpretation was smaller than without Sodium Fluoride administration as well as on 14th and 7th days. Sodium Fluoride can reduce osteoblast that is expressed by HSP-60 and MMP-8 in orthodontic teeth movement.