Pengaruh Naphthalene Acetic Acid (NAA) dan Kinetin terhadap Perkembangan Protocorm-Like Body (PLB) dan Regenerasi Anggrek Phalaenopsis sp. Hybrid

Abstract

Phalaenopsis is an orchid with high economic value due to its beautiful color. The demand of this orchid is increasing so that tissue culture technology is required in mass propagation of the orchid. Orchid propagation using a protocorm-like body (PLB) explant source is one of the best ways to produce large quantities of orchids. The study was aimed to identify the best combination of NAA and Kinetin in PLB development at liquid medium and the PLB that was subcultured in regeneration medium. The explant used was 2-month-old PLB that planted in liquid Vacin & Went (VW) medium with combinations of NAA and Kinetin at concentrations of 2.5 mg/L, 5 mg/L and 7.5 mg/L, respectively. The regeneration medium used was solid VW medium with supplements of 15% coconut water, 5% bananas, 5% potatoes and 0.2% activated charcoal. The experiment used a completely randomized design with three replications. The results showed that the combination of NAA and kinetin had no significant effect on the number and fresh weight of PLB. Meanwhile, almost all the combination showed the same colour of PLB, namely yellowish green (5GY 7/6), only the combination of NAA 5 mg/L + Kinetin 7.5 mg/L showed different result with pale yellow (5Y8 /4) colour. The plantlet colour change from light yellowish green (7.5GY 8/8) to yellowish green (7.5GY 7/6) and dark yellowish green (7.5GY 6/10) occurred at the beginning of acclimatization and at two and four weeks. Orchid seedlings grew well and became mature plants after being transferred to black moss media