Caffeine-Degrading Endosymbiont Fungi Isolated From Hipothenemus Hampei. Ferr as Pre-analysis Caffeine Toleranceability of Coffee Berry Borer

Abstract

The Coffee Berry Borer (CBB), Hypthenemus hampei Ferr., is the most destructivepest of coffee berry due to its ability to bore and complete their entire life cycle inside of thecoffee berry. The detoxification mechanism of CBB ability to lives inside of coffee berrycontaining high natural insecticide compound such as caffeine is a very interesting study and it may lead to be an advantaged for CBB controlling methods. Isolation and screening of endosymbiont fungi from CBB using 0.5% caffeine plate agar as a minimum nutrient as well asa selective medium were obtained 10 isolates consist of 6 isolates of yeasts and 4 filamentousfungi which positively degraded caffeine content through their formed diameter of the colony. The presenting of these several caffeine-degrading endosymbiont fungi could be as preexplanation about the caffeine tolerance mechanism of CBB.