Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/106524
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dc.contributor.authorHIDAYAT, Mochammad Amrun-
dc.contributor.authorMAHARANI, Diah Ayu-
dc.contributor.authorPURWANTO, Djoko Agus-
dc.contributor.authorKUSWANDI, Bambang-
dc.contributor.authorYUWONO, Mochammad-
dc.date.accessioned2022-04-22T01:41:52Z-
dc.date.available2022-04-22T01:41:52Z-
dc.date.issued2019-12-22-
dc.identifier.govdocKODEPRODI2210101#Farmasi-
dc.identifier.govdocNIDN0026017801-
dc.identifier.govdocNIDN0001026907-
dc.identifier.urihttp://repository.unej.ac.id/xmlui/handle/123456789/106524-
dc.description.abstractA simple paper-based colorimetric biosensor based on immobilized tyrosinase and 3-methyl-2-benzothiazolinone hydrazone (MBTH) was developed for assessing total polyphenol content (TPC) of various green tea beverages. The patterned biosensing zone of the paperbased biosensor showed a sensitive response to catechin (a typical green tea polyphenol) by generating pink color adducts that can be captured for further color image analysis using a scanometric method. This color change can be connected to the TPC of the samples. The analytical performance of the biosensor in scanometric set-up was optimized. The biosensor showed a response time of 13 min in the linear range between 0.08-1.03 mM of catechin (r = 0.9979). The detection limit (LOD) of the biosensor was 0.071 mM while reproducibility was found at 3.11% RSD (relative standard deviation). TPC of green tea beverages was assessed by the biosensor, and the results were in accordance with the UV/Vis spectrophotometric method.en_US
dc.language.isoenen_US
dc.publisherBiotechnology and Bioprocess Engineering volumeen_US
dc.subjectSensitive Paper-baseden_US
dc.titleSimple and Sensitive Paper-based Colorimetric Biosensor for Determining Total Polyphenol Content of the Green Tea Beveragesen_US
dc.typeArticleen_US
Appears in Collections:LSP-Jurnal Ilmiah Dosen



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