Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/106465
Title: Paper microzone plate based on DPPH as a simple colorimetric assay of the total antioxidant content of herbal extracts
Authors: KUSWANDI, Bambang
FANTONI, Muhammad
HIDAYAT, Mochammad Amrun
NINGSIH, Indah Yulia
Keywords: Paper microzone plate
Paper-based sensor
Colorimetric
DPPH
Total antioxidant capacity
Herbal extract
Issue Date: 3-Apr-2020
Publisher: Journal of Food Science and Technology
Abstract: The human body needs an antioxidant-rich diet that comes from foods, beverages, and herbal products to support the physiological antioxidant systems. Thus, the development of an analytical tool for a simple assay of the total antioxidant capacity (TAC) of the rich antioxidant samples is crucial. The current work demonstrates a simple colorimetric assay of TAC of the herbal extract on the paper microzone plate (PlZP) that was constructed in the 70-well of patterned paper using the screen printing technique. The PlZP was constructed by immobilizing DPPH (2,2-diphenyl-1-picrylhydrazyl) onto 70-well of PlZP as a sensing zone for colorimetric detection. The purple-sensing zone exhibited a good response to gallic acid (GA), by producing slightly gray to pale yellow color that can be captured using a scanner and then analyzed using the ImageJ program. The paper-based sensor showed a linear response toward GA at 0.05–0.6 mM (r = 0.9895), reproducible response (RSD \ 4%), and accurate measurement with 91 to 106% recovery for measuring TAC of herbal extract, presented as mM gallic acid equivalent and showed a good agreement with the standard DPPH method. The results suggested that the proposed method can be applied for simple TAC measurement in the herbal extract.
Gov't Doc #: KODEPRODI2210101#Farmasi
NIDN0001026907
NIDN0026017801
URI: http://repository.unej.ac.id/xmlui/handle/123456789/106465
ISSN: NIDN0012078401
Appears in Collections:LSP-Jurnal Ilmiah Dosen

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