Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/105922
Title: Micro-Computed Tomography Analysis on Administration of Mesenchymal Stem Cells - Bovine Teeth Scaffold Composites for Alveolar Bone Tissue Engineering
Authors: SARI, Desi Sandra
LATIEF, Fourier Dzar Eljabbar
FERDIANSYAH, Ferdiansyah
SUDIANA, Ketut
RANTAM, Fedik Abdul
Keywords: Mesenchymal Stem Cells
Bovine Teeth
Scaffold
Regeneration
Alveolar Bone
Periodontitis
Micro-computed tomography
3-Dimensional
Issue Date: 10-Aug-2021
Publisher: Journal of Biomimetics, Biomaterials and Biomedical Engineering
Abstract: The tissue engineering approach for periodontal tissue regeneration using a combination of stem cells and scaffold has been vastly developed. Mesenchymal Stem Cells (MSCs) seeded with Bovine Teeth Scaffold (BTSc) can repair alveolar bone damage in periodontitis cases. The alveolar bone regeneration process was analyzed by micro-computed tomography (µ-CT) to observe the structure of bone growth and to visualize the scaffold in 3-Dimensional (3D). The purpose of this study is to analyze alveolar bone regeneration by µ-CT following the combination of MSCs and bovine teeth scaffold (MSCs-BTSc) implantation in the Wistar rat periodontitis model. Methods. MSCs were cultured from adipose-derived mesenchymal stem cells of rats. BTSc was taken from bovine teeth and freeze-dried with a particle size of 150-355 µm. MSCs were seeded on BTSc for 24 hours and transplanted in a rat model of periodontitis. Thirty-five Wistar rats were made as periodontitis models with LPS induction from P. gingivalis injected to the buccal section of interproximal gingiva between the first and the second mandibular right-molar teeth for six weeks. There were seven groups (control group, BTSc group on day 7, BTSc group on day 14, BTSc group on day 28, MSCs-BTSc group on day 7, MSCs-BTSc group on day 14, MSCs-BTSc group on day 28). The mandibular alveolar bone was analyzed and visualized in 3D with µ-CT to observe any new bone growth. Statistical Analysis. Group data were subjected to the Kruskal Wallis test followed by the Mann-Whitney (p <0.05). The µ-CT qualitative analysis shows a fibrous structure, which indicates the existence of new bone regeneration. Quantitative analysis of the periodontitis model showed a significant difference between the control model and the model with the alveolar bone resorption (p <0.05). The bone volume and density measurements revealed that the MSCs-BTSc group on day 28 formed new bone compared to other groups (p <0.05). Administration of MSCsBTSc combination has the potential to form new alveolar bone.
Gov't Doc #: KODEPRODI1610101#Kedokteran Gigi
NIDN0015127804
URI: http://repository.unej.ac.id/xmlui/handle/123456789/105922
Appears in Collections:LSP-Jurnal Ilmiah Dosen

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