Lactobacillus Casei Fermentation Towards Xylooligosaccharide (Xos) Obtained from Coffee Peel Enzymatic Hydrolysate

Abstract

fee peel waste contains fibers including xylan, a polymer that constitutes hemicelluloses. Xylan can be extracted from coffee peel and used for xylooligosaccharide (XOS) production. XOS is an oligomer that has the potentials as a prebiotic. XOS production could be carried out by enzymatic hydrolysis using endo-β-1,4-d-xylanase. In this research, the XOS from coffee peel was investigated for its prebiotic potential on the growth of Lactobacillus casei (L.casei) in-vitro. The assay was carried out by adding various concentrations of XOS to the bacterial culture media under different incubation times. L. casei growth was calculated based on the formed bacterial colonies log CFU/mL. Addition of 20% XOS to 10 mL of L. casei growth media showed the best result at 12 h with 8.75 log CFU/mL. Increased bacterial colonies proved that XOS in fermented media was beneficial for the bacteria, resulting in bacterial cell proliferation. Other parameters observed in this study were the decreased pH value, the reduced XOS level and the increased content of organic acids in the bacterial culture media.