Immunodetection of Adhesin Pili Protein 38.6 Kda K. Pneumoniae Using Western BLOT

Abstract

Klebsiella pneumoniae is a pathogen that causes infections in communities and hospitals. These bacteria have many virulence factors that play an important role in the pathogenicity of infection and antibiotic resistance. Pili protein Klebsiella pneumoniae with 38.6 kDa as one of the virulence factors that have roles as hemagglutinin dan adhesin protein so potentially as a vaccine candidate. This research aimed to immunodetection pili protein 38.6 kDa using Western Blot. Western Blot was used to detect that protein with specific primary antibodies. These antibodies are obtained from mice serum-induced intraperitoneally with Pili protein 38.6 kDa Klebsiella pneumoniae. Protein bands that appear on the membrane of Western Blot results are proteins with a molecular weight of 85.6 kDa, 65.5 kDa, 46.9 kDa, and 29.4 kDa. This study concludes that Pili protein 38.6 kDa Klebsiella pneumoniae as the target protein does not appear in the Western Blot result