Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/105505
Title: Efect of ethylene inhibitor, type of auxin, and type of sugar on anther culture of local East Java aromatic rice varieties
Authors: ARISANDI, Dewi Puspa
PARADISA, Fragaria Vesca
SUGIHARTO, Bambang
AVIVI, Sholeh
FANATA, Wahyu Indra Duwi
Keywords: Aromatic rice
Anther culture optimization
Double haploid
Issue Date: 12-May-2020
Publisher: Journal of Crop Science and Biotechnology
Abstract: Breeding using the double haploid approach has been used in accelerating the rice breeding process. However, the main obstacle in the application of this technique in rice is the low level of callus formation and their regeneration into haploid plants. Based on these issues, this research is aimed at developing the best method for obtaining double haploid plants through anther culture using three kinds of aromatic rice of Indonesian local varieties. Anthers of Pendok, Genjah Arum, and Merah Wangi varieties were cultured in callus induction media which were either supplemented with diferent types of carbon sources, or auxins and ethylene inhibitors. However, the application of diferent sugar types did not signifcantly afect callus formation of Pendok and Genjah Arum. However, auxin treatment showed NAA to be more efcient for the callus induction of Pendok, while 2,4-D had no efect on callus induction in all tested varieties. The addition of ethylene inhibitor successfully induced callus formation of Merah Wangi varieties, while the rates of Pendok and Genjah Arum were not signifcantly afected. Callus of three plant lines were then regenerated into plantlet in regeneration media. Of the three callus varieties used, only Pendok could regenerate into plantlets and grow into adult haploid plants.
URI: http://repository.unej.ac.id//handle/123456789/105505
ISSN: Kodeprodi#1520101#MagisterAgronomi
NIDN#0021076901
NIDN#0022105504
NIDN#0004028106
Appears in Collections:LSP-Jurnal Ilmiah Dosen



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