Chemical Composition Of Kasturi Tobacco Resinoid Determined By Gas Chromatography-mass Spectrometry

Abstract

In Indonesia, Kasturi tobacco is widely cultivated in Jember and Bondowoso Districts. This local tobacco planted in dry season and sundried processed which the leaves were used as the raw material of premium cigarettes. The leaves constitute of diverse molecules with various biological or pharmacological activities that have been widely used in medical treatments in form of natural based medicament. This study aimed to determine the chemical composition in the Kasturi tobacco leaves using gas chromatography-mass spectrometry (GC-MS). Kasturi tobacco leaves were air-dried prior extraction using stirred assisted maceration method for 24 hours in methanol. Less polar components was extracted using n-hexane and vacuum dried before fractionation under a silica column chromatography. Fractions were analyzed using gas chromatography-mass spectrometry resulted in detection of esters, hydrocarbons, alcohol, nitrogenous compounds and terpenoids including, two unique tobacco resinoids α-CBT (4,8,13-cyclotetradecatriene-1,3-diol) and the labdanoid (11E, 13Z)-labdadien-8-ol and (12Z)-abienol.