Catechin Isolated from Green Tea GMB4 Clone Promotes Expression of Nrf2 in Experimental Cataract

Abstract

Purpose: This research was aimed to show the nuclear factor-erythroid-2- related factor 2 (Nrf2) in seleniteinduced cataract. Method: Cataract was formed by inducing 19 µmol/kg sodium selenite subcutaneous to ten days-old Wistar rats (Neonates). Catechin induction were given into neonatal rats with five different experimental groups based on the four different catechin doses (0, 50, 100, 200 mg/kg) and one group of control. Catechin was isolated from GMB4 Clone Green Tea. The Nrf2 in cataract was examined using slitlamp biomicroscopic analysis and immunohistochemistry. Results: Neonatal rats in group I did not form cataracts on both eyes. In Group 2, there are one of five (20%) neonatal rats that experienced stage three cataracts, and the remaining four out of five (80%) stage four cataracts. In groups III, IV, and V, neonatal rats experienced a decrease in cataract formation. There was a significant difference in cataracts in each stage in all rats from Group II (P = 0.022, 0.001, 0.001). The average number of Nrf2 group II (P < 0.01) was lower than in Group I, Group III, Group IV, and Group V (P= 0.000; 0.006; 0.003; 0.000). Conclusions: Nrf2 increased gradually followed by the decreasing opacity of the lens by catechin isolated From GMB4 Clone Green Tea. Catechin dose of 200 mg/kg BW promotes the Nrf2 in experimental cataract.