PERBANDINGAN JUMLAH KOLONI BAKTERI PLAK SUBGINGIVA PADA MASA PRAPUBERTAS, PUBERTAS DAN PASCAPUBERTAS

Abstract

Dental Plaque is a non-calcified soft material which is strongly bond onto the teeth surface and is resistant to the cleaning action from the flow of saliva. About 70% of the plaque’s volume consists of bacteria cells. This plaque bacterium is the main etiology factor which causes dental caries and periodontal diseases. In the case of periodontal disease, it was thought that subgingiva plaque bacteria could penetrate into the pocket or epithelium junctional which causes further damage to periodontal tissues. This research aims to compare the amount of subgingiva plaque bacteria during prepuberty, puberty and postpuberty. Total of 18 subjects were sampled which were divided into three groups. Each of the subjects was taken the subgingiva plaque on the Molar 1 bucal surface of the upper jaw. The plaque was liquefied by 10-2 and inoculated into the TSA medium using pour plate technique. It was then incubated at 37oC temperature for 24 hours and the counting of subgingiva plaque bacteria was performed using Colony Counter. In average, the amount of subgingiva plaque bacteria colony obtained during prepuberty was 189 cfu, during the puberty was 376.83 cfu and postpuberty was 255.50 cfu. After that, the ANAVA test was carried out with the confidence level of 95% and the meaningful difference was observed (p<0.05). Finally, Turkey HSD test was performed and the meaningful difference was observed in all pairs (p<0.05). It is concluded that the subgingiva plaque bacteria was observed most during the puberty, followed by postpuberty and prepuberty periods. During the puberty period, there’s increase in the amount of subgingiva plaque bacteria colony which is suspected to have been caused by change in the hormone balance. Some researches revealed that the increase in the amount of estrogen and progesterone during pregnancy and puberty could increase the dental plaque bacteria.