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dc.contributor.authorHi d ayah M u r t iyan in gs ih
dc.date.accessioned2014-01-22T01:28:46Z
dc.date.available2014-01-22T01:28:46Z
dc.date.issued2014-01-22
dc.identifier.nimNIM081810401023
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/20389
dc.description.abstractS ukr o s a m e r upa ka n pr o duk ut a m a f o t o s i n t e s i s ya n g d i ha s il ka n d a r i pr o s e s a s i mi l a s i ka r b o n . S ukr o s a di s i n t e s i s d i s i t o s o l y a n g d i ka t a l i s i s o l e h e n z im Suc r os e P hos phate S y nthas e (S PS) . S ukr o s a y a n g d i h a s i l ka n d i t r a n s po r t ke or ga n pe nyim pa n a n da n d i gu n a ka n s e ba ga i s u m be r e n e r g i u n t uk pe r t um b u ha n da n pe r ke m b a n ga n t a n a m a n . T r a ns po r t s ukr o s a t e r s e b ut di f a s il i t a s i o l e h pr o t e i n Su c r o s e T r ans por te r ( S UT ) y a n g t e r l e t a k d i m e m b r a n s e l . B e s a r ny a ka n du n ga n s ukr o s a pa da o r ga n pe nyim pa n a n s a l a h s a t uny a d i pe n g a r uhi o l e h t i n gka t tr a n s po r t a s i ny a . A kt i vi t a s t r a n s po r t s ukr o s a m e r upa ka n h a l p e n t i n g u n t uk m e mi nda h ka n s ukr o s a da r i o r ga n f o t o s i n t e s i s ( s ou r c e ) ke o r ga n n o n f o to s i n t e s i s ( s i nk ) . P e n e li t i a n i n i b e r t u j ua n u n t uk m e n ge t a h u i ka r a kt e r pa da t a n a m a n t e b u t r a n s ge ni k o v e r e ks pr e s i ge n SoSUT1 , y a n g b e r h u b u n ga n de n ga n e k s pr e s i g e n SoSUT1 y a n g t e r i n s e r s i ke da l a m ge n o m t a n a m a n t e b u . K a r a kt e r i s a s i y a n g d il a kuka n m e li put i a na l i s i s DN A ge n o m de n ga n P C R , a n a l i s i s pr ot e i n S UT 1 da n S P S 1 de n ga n w e s t e r n blot , da n a n a li s i s ka n du n ga n s ukr o s a . K o nf i r m a s i ke b e r a da a n ge n SoS UT1 pa da t e b u t r a ns ge ni k d il a kuka n m e n ggu n a ka n a n a li s i s P olym e r as e C hain R e ac t ion ( P C R ) de n ga n m e nggu n a ka n pr i m e r hy gr omic in pho s pho tr an s f e r as e II ( hptI I ) , da n d il a kuka n pa d a 28 e v e nt t a n a m a n t r a n s ge ni k h a s i l pe r b a ny a k a n s e c a r a s t e k b a t a n g. An a li s i s pr o t e i n S UT 1 da n S P S 1 di l a kuka n de n ga n m e t o de w e s te r n blot un t uk m e n ge t a h u i e ks pr e s i ge n SUT1 pa da t i n gka t t r a n s l a s i . P a da pe n e li t i a n i ni j uga d il a kuka n a n a li s i s s ukr o s a pa da o r ga n da un da n b a t a n g t e b u , u n t uk m e n ge t a h u i pe n ga r u h ge n SoSUT1 y a n g t e l a h t e r i n s e r s i vii t e r h a da p ka n du n g a n s ukr o s a ba t a n g t e b u y a n g mer upa ka n o r ga n pe nyim pa n a n pa da t a n a m a n t e b u. Ha s il a na l i s i s P C R de n ga n pr i m e r hptI I m e n u njukkan b a h wa da r i s e m ua t a n a m a n y a n g d i a na l i s i s , t e r da pa t 13 t a n a m a n t e b u po s i t i f t r a n s ge ni k SoSUT1 de n ga n ukur a n a m p l if i ka s i 470 b p, s e da n gka n 15 t a n a m a n l a i nn y a m e n u nj ukka n h a s il n e ga t i f . Ha s il a n a li s i s w e s te r n blot pr ot e i n s uc r os e tr an s po r ter 1 ( S UT 1 ) t e r h a da p 13 t a n a m a n t r a n s ge ni k m e n u nj ukk a n t e r da pa t 9 e v e nt t a n a m a n y a ng m e n ga l a mi pe ni ngka t a n ka n du n ga n pr ot e i n , d i t a n da i de n ga n t e b a l n y a p i t a pr ot e i n d i ba n d i ngka n de n g a n ko n t r o l . An a li s i s pr ot e i n s uc r os e phos phate s y nt has e 1 ( S P S 1) m e n u nj ukk a n b a h wa t a n a m a n t r a n s ge ni k m e mi li k i k a n du n ga n pr o t e i n y a ng c e n d e r un g s a m a de n ga n ko n t r o l . Ha s il a n a li s i s s ukr o s a da u n m e n u nj ukka n b a h w a pa da s e m ua e v e nt t a n a m a n t r a n s ge ni k m e mi li k i pe r s e n t a s e ka n du n ga n s ukr os a l e bi h r e n d a h d i ba n d i n gk a n t e b u k o n t r o l . Ha s i l a n a li s i s s ukr o s a b a t a n g p a da r ua s ke 3 da n ke 8 m e n g a l a m i pe ni ngka t a n pe r s e n t a s e ka n du n ga n s ukr o s a a pa bil a d i ba n d i ngka n de n ga n t a n a m a n ko n t r o l .en_US
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dc.relation.ispartofseries0 81810401023;
dc.subjectK ar ak t e r is a s i T an a m an T e b u ( S ac c h ar u m of f i c in ar u m L . Var . B L ) T r an s g e n ik Over e k s p r e s i Gen S oS UT 1 E v e n t A D ;en_US
dc.titleK AR A K T E RI S ASI T AN AM AN T E B U ( S ac c h a r u m o f f icin ar u m L . Var . B L ) T RA NSG E NI K OVE RE K S P RE S I GE N S oS UT 1 E V E NT A Den_US
dc.typeOtheren_US


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