Analisis Respon Imun Humoral (IgG) Mencit (Mus musculus) Pasca Paparan Fraksi Protein 31 kDa Kelenjar Saliva Aedes aegypti

Abstract

Dengue virus can cause the Dengue Hemorrhagic Fever (DHF) is an infectious disease in tropical and subtropical areas. This fever caused by one of the serotype of Dengue virus (DENV-1, DENV-2, DENV-3, or DENV-4). Dengue virus is transmission when Ae. aegypti blood feeds to the host. Blood feeding process is facilitated by protein in the salivary glands of the Ae. aegypti. This study used 31 kDa protein from salivary glands Ae. aegypti. Protein 31 kDa had the main component D7 which can initiate an immune response in the host or human body (immunomodulatory). This study aims to analyze the humoral immune response (IgG) of mice (Mus musculus) after repeated exposure to the 31 kDa immunogenic protein in the salivary glands of Ae aegypti.This study began with landing collection and rearing of Ae. aegypti, isolation of salivary glands, analysis of salivary glands protein using SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), purification protein using electroelution, animal treatment, preparation of blood serum, and IgG level measurment using ELISA (Enzymelinked Immunosorbent Assay). IgG level then analysis using SPSS (Statistical Product and Service Solution) with One Way Anova test and DMRT test (α= 0.05). The result show that group C (31 kDa protein (0,2 µg/µl) + adjuvant) had the highest IgG levels than group A (buffer elusi) and group B (adjuvant). These results were strengthened by statistical tests with a significance value in the 2nd week, p = 0.020 < 0.05, and in weeks 4 and 6 the value obtained was p = 0.000 < 0.05 in individual samples and p = 0.025 > 0, 05 in population sampels. Thus repeated exposure to the 31 kDa salivary gland protein Ae. aegypti can increase IgG concentrations. This proves that the 31 kDa protein is immunogenic and capable of inducing the host's immune response.