The Adhesion Activity to Monocytes Against Streptococcus mutans with Exposure Steeping Robusta Coffee Beans
Dewanti, I Dewa Ayu Ratna
Yani, Ristya Widi Endah
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Aim and Objectives: The aim of the study is to analyze the adhesion activity to monocytes against Streptococcus mutans with exposure steeping robusta coffee beans in vitro. Materials and Methods: Peripheral blood sampling of healthy people 6 cc added anticoagulants. Peripheral blood sampling of healthy people 6 cc added anticoagulants and Ficoll‑Hypaque, then centrifugation and suspended in medium Roswell Park Memorial Institute (RPMI) 1640. The cells were placed in 96‑well microtiter plate 8 × 105 cells/well for 45 min at 37°C and washed 4 times with the medium. Attached is a monocyte. Furthermore, the isolate of monocytes and treated with the appropriate group. The control group – monocytes untreated; S. mutans group – monocytes + S. mutans; coffee group 2.5% – steeping coffee beans 2.5% + S. mutans; coffee group 5% – steeping coffee beans 5% + S. mutans; and coffee group 10% – steeping the coffee beans 10% + S. mutans. After incubated 24 h, fixed with methanol, performed Giemsa staining, and analyzed adhesion activity of under a light microscope With magnification 400 × on to 100 monocytes. Data were analyzed using analysis of variance (ANOVA) followed by least significant difference (LSD) test. Results: There were significant differences between the study groups. Steeping robusta coffee beans improve adhesion S. mutans in monocyte cells, while the largest adhesion at a concentration of 5%. Conclusions: Certain concentrations of steeping robusta coffee beans consumption increase adhesion activity to S. mutans.
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