| dc.description.abstract | Tithonia diversifolia has been showed to be cytotoxic and antiproliferative on colon
cancer, glioblastoma, hepatoma, kidney cancer, breast cancer, lung cancer, melanoma,
leukemia, ovary cancer, prostate cancer, and stomach cancer cell lines, but not on cervical
cancer cells yet. Our research aimed to determine the cytotoxicity and antiproliferative
activity of T.diversifolia leaf ethanolic extract on HeLa cervical cancer cell line. The
cytotoxicity and the antiproferative activity assay were done using MTT method for
24 h for cytotoxic assay; and series of 24, 48, and 72 h for antiproliferative assay. The
cytotoxic activity was analyzed using IC50, while the antiproliferative assay was analyzed
based on the proliferation kinetics. All assays were done in triplicate. T.diversifolia leaf
ethanolic extract exhibited strong cytotoxic activity on HeLa cervical cancer cell lines with
the IC50 of 97.839±10.120 µg/mL. The cytotoxic activity was dose dependent. Based on
the proliferation assay, the antiproliferative activity was stronger as the incubation time
and the dose increases. T.diversifolia leaf ethanolic extract showed strong cytotoxic and
antiproliferative activity on HeLa cervical cancer cell lines. | en_US |
