| dc.description.abstract | This study was to investigate the effect of Physalis angulata L. leaf water extract on vascular rarefaction, oxidative stress, and inflammation
on the kidney of Nω-nitro-L-arginine methyl ester (L-NAME)-induced male Wistar rats.
Methods: A total of 25 male Wistar rats were divided into five equal groups (normal control: 40 mg/kg/day of normal saline; L-NAME group; and treatments I,
II, and III: L-NAME plus P. angulata L. leaf water extract doses 500 mg/kg/day, 1500 mg/kg/day, and 2500 mg/kg/day, respectively). Endothelial dysfunction
was induced by 40 mg/kg/day L-NAME intraperitoneally. The treatment lasts for 15 days. Vascular rarefaction was indicated by the decrease in vascular
density, which considered as vascular number per high-power field in hematoxylin-eosin staining preparation. Kidney oxidative stress test was performed
by measuring malondialdehyde (MDA) level with thiobarbituric acid reactive substances assay. The inflammatory marker was the nuclear factor kappa-lightchain-enhancer of activated B cells (NF-κB) expression which examined using an immunohistochemical method with an antibody against p65.
Results: At the dose of 500 mg/kg/day and 1500 mg/kg/day, P. angulata leaf water extract supplementation increased the vascular density, decreased
the MDA level, and decreased the NF-κB expression compared to the L-NAME group.
Conclusion: The administration of P. angulata L. leaf water extract in particular concentration has a vasoprotective effect by preventing kidney
vascular rarefaction, oxidative stress, and inflammation on L-NAME-induced male Wistar rat. | en_US |
