Re-design and Exploration of Extraction of Phenolic Compounds from Sargassum Sp using Ethyl Acetate as Solvent

Abstract

A Marine brown algae, Sargassum sp, is well-known as a bioactive compound. One of the most important compounds is polyphenol substance. This research is ongoing to obtain the highest yield soluble extract of polyphenol. In spite of ethanol, ethyl acetate is one of the biobased solvents which is strongly considered as safe for food and health supplement product. The objective of this study is to define the optimum condition to extract phenolic content from Sargassum sp. using ethyl acetate. The maceration method was conducted by pouring the ethyl acetate into the flask containing Sargassum Sp powder. Since the extraction time is completed, the extract was separated and purified. The total phenol was quantitatively determined using the folin ciocalteau method. The constituent of extract was checked qualitatively by thin-layer chromatography.To define the optimum condition, response surface method based on central composite design was employed. The process variables in this experiment are particle size (60-100 mesh), ratio Sargassum Sp to solvent (1:2-1:4 gr/mL) and extraction time (1-3 days). This work concludes that optimum condition is achieved at a particle size of 100 mesh, extraction time of 3 days and ratio Sargassum to solvent of 1:4 g/mL. This optimum condition provides the highest yield (47 wt %) as well.