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dc.contributor.authorSawitri, Widhi Dyah-
dc.contributor.authorSugiharto, Bambang-
dc.date.accessioned2020-01-20T07:55:07Z-
dc.date.available2020-01-20T07:55:07Z-
dc.date.issued2019-01-09-
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/97044-
dc.descriptionThe 12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry (CoSCI)" and AnMicro Workshop 11-12 October 2018, Universitas Airlangga, Indonesiaen_US
dc.description.abstractSucrose occupies many essential roles to control regulation of carbon partitioning in plants, including prokaryotic cells. Sucrose phosphate synthase (SPS; EC 2.4.1.14) is a key enzyme to catalyze the form of sucrose in primary sucrose synthesis pathway. Plants SPS has a molecular size around 120 kDa, which consists of N-terminal domain, C-terminal domain, and central domain. We produced the recombinant sugarcane SPS (SoSPS1) in Escherichia coli, however, the expression often appears to be a shorter form with retained enzyme activity. In our result, we reported that the shorter form is suggested to have a truncated N-terminal 20-kDa region. The truncated form of SoSPS1 (∆N-SPS) tends to enhance the specific activity 10-fold compared to full-length SoSPS1. The full-lenght SoSPS1 showed a remarkable allosteric activation by glucose-6-phosphate (G6P), while none of the N-terminal truncated form had such a characteristic. By kinetic analysis of full-length SoSPS1, a higher substrate affinity was shown in the presence of G6P. Conversely, the ∆N-SPS showed a similar substrate affinity whether G6P was added or not. Based on these results, we revealed that N-terminal region of SoSPS1 has essential role for allosteric regulation by G6P and may function like a suppressor domain for the enzyme activity.en_US
dc.language.isoenen_US
dc.publisherIOP Conf. Series: Earth and Environmental Science 217 (2019) 012043en_US
dc.subjectsucrose phosphate synthaseen_US
dc.subjectN-terminus deletionen_US
dc.subjectrecombinant enzymeen_US
dc.subjectsugarcaneen_US
dc.titleRevealing the Important Role of Allosteric Property in Sucrose Phosphate Synthase from Sugarcane with N-terminal Domain Deletionen_US
dc.typeArticleen_US
dc.identifier.kodeprodiKODEPRODI2520101#Magister Bioteknologi-
dc.identifier.nidnNIDN0022105504-
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