Pengembangan dan Validasi Metode KLT Densitometri untuk Penetapan Kadar Kurkumin dalam Ekstrak Etanol Rimpang Temulawak (Curcuma xanthorrhyza)

Abstract

A simple, sensitive, selective and specific, precise and accurate TLC densitometry method has been developed and validated for determination of curcuma xanthorrhyza extracts. The method employed TLC silica gel 60 F254 as the stationary phase and using chloroform : ethanol: glacial acetic acids (94:5:1 v/v/v) as mobile phase. Quantitative evaluation was performed by measuring the absorbance of the analyte spot at 424 nm. The linear regression data for the calibration plots of curcumin showed good linear relationship with r = 0,9967 in the concentration range 30-120 ppm of curcuminoid. The minimum detectable amounts were found to be 58,7ng curcumin/spot and the limits of quantitation were found to be 169,2 ng curcumin/spot. Repeatability precision, as RSD was 0,107% and Intermediet precision, as RSD was 0,14%. This TLC Densitometry method is selective and specific, precise, and can be used for routine analysis of curcumin.