Please use this identifier to cite or link to this item: https://repository.unej.ac.id/xmlui/handle/123456789/89893
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMuflihah, Yeni Maulidah-
dc.contributor.authorZulfikar, Zulfikar-
dc.contributor.authorSiswoyo, Siswoyo-
dc.contributor.authorAsnawati, Asnawati-
dc.contributor.authorAyun, Qurrota-
dc.date.accessioned2019-03-27T09:41:24Z-
dc.date.available2019-03-27T09:41:24Z-
dc.date.issued2019-03-27-
dc.identifier.isbn978-602-60569-5-5-
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/89893-
dc.descriptionProceeding The 1st IBSC: Towards The Extended Use Of Basic Science For Enhancing Health, Environment, Energy And Biotechnology (2016)en_US
dc.description.abstractHigh Performance Liquid Chromatography (HPLC) method combine with potentiometric detector was applied as a method for analysis of amino acids (aspartic acid, glutamic acid and arginine). The separation was carried out on a C18 column with isocratic elution of a mixture of acetonitrile and phosphat buffer. Potentiometric detector using a tungsten oxide electrode as working electrode and Ag/AgCl as refference electrode. The detection method was based on the presence of H+ ions from amino acids. This application was a development of previous studies that was succesfully applied to detect the presence of amino acids in batch and flow systems. Amino acid determination was characterized by its retention time. Retention time of aspartic acid, glutamic acid and arginine were as follow: 8,46; 13,0; and 15,21 minutes. The optimized separation conditions obtained at a flow rate of 1,2 mL/min with 15% acetonitrile concentration, buffer pH 6,5 with phosphat concentration of 5x10-4 M. Detector performance tested by the recovery test of samples, and the results obtained for glutamic acid, aspartic acid and arginine respectively: 89,1%, 94,9%, and 110%. Linear range obtained at 10-3 M to 10-7 M. Detection limit were 1,58x10-7 M for glutamic acid, 6,58x10-8 M for aspartic acid and 6,51x10-8 M for arginine.en_US
dc.language.isoenen_US
dc.subjectamino aciden_US
dc.subjectHPLCen_US
dc.subjecttungsten electrodeen_US
dc.subjectpotentiometryen_US
dc.titleHigh Performance Liquid Chromatography of Amino Acids Using Potentiometric Detector With A Tungsten Oxide Electrodeen_US
dc.typeProsidingen_US
Appears in Collections:LSP-Conference Proceeding

Files in This Item:
File Description SizeFormat 
F. MIPA_Prosiding_Siswoyo_High Performance Liquid Chromatography.pdf2.49 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.