Analisis Profil Protein Ekstrak Aquades dan Etanol Daun Mimba (Azadirachta Indica A. Juss) dengan Metode SDS-PAGE

Abstract

Background: Neem leaf is used to increase body immune response, antivirus, antifungal, antibacterial, and antioxidant. These biology activities are suspected because of protein in neem leaf. Protein in plants can be used as pharmacogenetic identification because some proteins in plants posse characteristic can defense to some pathogen microorganism. The way to know protein in the organism is using profile protein analytic through SDS-PAGE method. SDS-PAGE separates protein molecules by size, particular shape and also can determine protein molecular weight. Protein profiles can be obtained with protein extracts using solvents. Protein is a polar compound which is bonded to a polar solvent therefore chosen ethanol and aquadest as a liquid as extraction solvent. Purpose: To know the protein profile of aquadest and ethanol extracts of neem leaf by using SDS-PAGE. Method: This study used neem leaf as a sample, aquadest and 70% ethanol as the extraction solvent then analyzed using SDS-PAGE. Result: The results showed 9 protein bands on the aquadest extract of neem leaf and 8 of protein bands on the ethanol extract of neem leaf. Conclusion: From the study, there was a difference between the amounts of protein bands aquadest extracts of neem leaf with ethanol extract of neem leaf.